论文 · 体外 / 类器官研究
测量肠道细胞和类器官中脂肪酸吸收及细胞内脂质蓄积的方案
Protocol for measuring fatty acid absorption and intracellular lipid accumulation in intestinal cells and organoids
作者:Jiaqi Wu, Yu Gu, Shuangshuang Liu, Shupei Wang, Li-Hao Huang
STAR Protoc · 2026年9月23日 · Wu 等 5 位作者
不需要生物学背景,多打比方
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摘要Abstract
Quantitative assessment of intestinal epithelial cells is essential for studies of metabolism and disease. Here, we present a protocol to quantify fatty acid uptake and lipid accumulation in intestinal epithelial cell lines and primary intestinal organoids using a flow cytometry-based approach with BODIPY FL C16. We describe steps for assessing fatty acid uptake and accumulation in intestinal epithelial cell lines and intestinal organoids. This rapid and reproducible technique can also be adapted to investigate fatty acid metabolism in other cell types. For complete details on the use and execution of this protocol, please refer to Wu et al.1.
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