节肢动物的感染性转基因技术
Infectious transgenesis in arthropods
bioRxiv · 2026 年 9 月 17 日 · Najmeh Nejat, Travis van Warmerdam, Jason L Rasgon
过去十年，节肢动物的体内生殖系基因编辑技术快速发展，最早由ReMOT Control方法开创，后续DIPA-CRISPR和SYNCAS等技术在很多物种中实现了基因敲除，但稳定的基因插入一直难以实现，限制了广泛应用。研究者开发了基于杆状病毒AcMNPV的新系统，通过口服喂食将可遗传的CRISPR敲除和敲入构件递送到节肢动物生殖系，命名为“病毒转导生殖系工程”（GEValT）。新羽化的雌雄成虫喂食含病毒的糖水（和/或血液）后交配，即可方便地获得基因修饰后代。GEValT操作简便，可能改变多种节肢动物物种的基因编辑方法学。尚未经同行评审。
为什么推荐给您：口服病毒递送实现节肢动物可遗传敲入的新平台，填补该领域稳定基因插入的技术空白。
不需要生物学背景，多打比方
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摘要Abstract
In the last decade, there has been an explosion in the development of in vivo germline genetic editing technologies in arthropods, first pioneered by the Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) method. While ReMOT Control and related technologies (DIPA-CRISPR and SYNCAS) have shown great utility for gene knock-out in many different species, consistent gene insertion has been elusive, limiting widespread adoption. We developed a novel system based on the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) to deliver heritable CRISPR knock-out and knock-in constructs to the arthropod germline by oral feeding. We call this method "Germline Engineering by Viral Transduction" ("GEValT"). With GEValT, newly-eclosed female and male adult arthropods are fed on virus in sugar (and/or blood) and allowed to breed, resulting in easy generation of genetically modified offspring. GeValT is easy to use and has the potential to revolutionize gene editing methodology for researchers working across diverse arthropod species.