超低成本开源常温离体器官灌注平台的开发与验证
Development and Validation of an Ultra-low-cost, Open-source Normothermic Ex Vivo Organ Perfusion Platform
常温机器灌注（在体温条件下用血液样液体持续给离体器官供氧供营养）有望推动器官保存、治疗探索和疾病建模，但商业设备昂贵且难以获得，限制了研究。研究者用回收、再利用和低价元件搭建了一套灌注回路，先用猪肾和自体血反复优化，再通过猪肾自体移植验证移植后的肾功能，并用人类血制品灌注不适合移植的人类供体肾、脾和胰腺。系统成本约2000美元，可灌注猪肾和人类器官长达24小时并保持功能和活性，关键技术包括允许非肝素化器官低流量灌注的机制和带可调磁铁的固定袋。移植后的猪肾在体内有功能，存活30天。作者认为这一可扩展的低成本平台为离体研究提供了可行路径。
为什么推荐给您：以极低成本开源平台实现常温器官灌注并完成在体移植验证，有望大幅降低该技术研究门槛。
不需要生物学背景，多打比方
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摘要Abstract
BACKGROUND: Normothermic machine perfusion (NMP) promises to catalyze organ preservation, therapeutic discovery, and disease modeling. Commercial organ perfusion devices remain inaccessible for research because of restricted access, high costs, and steep technical learning curves.
METHODS: To overcome these barriers, an NMP circuit was developed using recycled, repurposed, and low-cost components. Porcine kidneys and autologous blood were used for iterative optimization. A porcine kidney autotransplantation protocol was adapted to evaluate in vivo kidney function after NMP. Nontransplantable human donor kidney, spleen, and pancreas specimens were perfused using human blood products.
RESULTS: This effort resulted in an ultra-low-cost (~$2000) NMP system capable of perfusing porcine kidneys and human organs (kidney, pancreas, and spleen) for up to 24 h with evidence of both function and viability. Key innovations included a mechanism to permit low-flow perfusion in nonheparinized organs and a containment bag with adjustable magnets to provide stabilization. Porcine kidneys perfused on this platform demonstrated in vivo function upon autotransplantation with survival for 30 d.
CONCLUSIONS: We conclude that an ultra-low-cost NMP platform can be constructed from readily available materials. This financially sustainable and scalable model provides a road map to advance ex vivo discovery.