牛胎儿脂肪组织来源间充质干细胞与睾丸类器官中生殖细胞标志物表达的关联

Organoids serve as three-dimensional structures that offer spatial support and mimic cellular niches for experimental studies. The spatial and temporal association of fetal bovine adipose tissue-derived mesenchymal stem/stromal cells (AT-MSCs) with the expression of germ cell (GC) markers was evaluated in testicular organoids (TOs+AT-MSCs) under the effect of retinoic acid and testosterone (0.2, 2 and 20 µM). TOs+AT-MSCs were developed by the co-culture of Leydig, Sertoli, and peritubular myoid cells and PKH26+AT-MSCs and were analyzed for the expression of DAZL, STRA8 and UCHL1. TOs+AT-MSCs treated with 1 µM of retinoic acid and 2 or 20 µM of testosterone maintained their diameter after day 7 of culture. Pearson correlation coefficients (PCCs) of PKH26 and DAZL were higher (p < 0.05) at days 7 and 21 in TOs+AT-MSCs treated with 1 µM retinoic acid and 0.2 or 2 µM of testosterone, respectively. PCCs of PKH26 and STRA8 or UCHL1 were higher (p < 0.05) at days 14 and 21 in TOs+AT-MSCs treated with 1 µM retinoic acid and 0.2 or 2 µM of testosterone. Thus, exposure to 1 µM of retinoic acid and 2 µM of testosterone resulted in a similar diameter with respect to the control and spatial and temporal association of AT-MSCs and DAZL, STRA8 and UCHL1 markers in TOs+AT-MSCs.